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fusA [2019-08-16 10:50:46]
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fusA [2019-08-16 10:50:46]

elongation factor G, facilitates movement of tRNA and mRNA by one codon
locus
BSU_01120
pI
4.61
mw
76.36 kDa
protein length
692 aa Sequence Blast
gene length
2079 bp Sequence Blast
function
translation
product
elongation factor G
essential
yes
synonyms
fus
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

    • Gene

    Coordinates
    130,684 132,762

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • hydrolyses GTP

    • Protein family

  • [SW|TRAFAC class translation factor GTPase superfamily] (according to UniProt)
  • [SW|Classic translation factor GTPase family] (according to UniProt)

    • [SW|Domains]

  • tr-type G domain (aa 8-282) (according to UniProt)

    • Modification

  • phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) [Pubmed|17218307], [Pubmed|17726680]
  • phosphorylated on Tyr-339 [Pubmed|26381121]

    • Structure

  • [PDB|1ELO] (from ''Thermus thermophilus'') [Pubmed|8070397]
  • [PDB|4BTC], [PDB|4BTD], the ''Thermus thermophilus'' EF-G[SW|ribosome] complex in a pretranslocation state [Pubmed|23912278]

    • [SW|Localization]

  • cytoplasm (according to UniProt)

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

    • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the mRNA is processed between [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB] and [gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
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    Biological materials

    Expression vectors

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP840, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP848, available in [SW|Jörg Stülke]'s lab

    • References

  • 24614227,17726680,17218307,20348921,8070397,23912278,15378759,25108354,26381121,27524615,26072700